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yhfP [2018-09-26 09:29:37]

similar to predicted oxidoreductase, Zn-dependent and NAD(P)-binding

locus

BSU10320

4.72

34.60 kDa

protein length

330 aa Sequence Blast

gene length

990 bp Sequence Blast

function

unknown

product

unknown

essential

synonyms

Outlinks

KEGG BsubCyc SubtiList UniProt Expression Browser

Genomic Context

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categories

[category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates

1,106,524 → 1,107,516

The protein

Protein family

[SW|zinc-containing alcohol dehydrogenase family] (according to UniProt)

[SW|Cofactors]

NADP⁺ (according to UniProt)

Structure

[PDB|1TT7] (99% identity)

[PDB|1Y9E] (with NAD), [PDB|1TT7]

[SW|Localization]

cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

genes

description

[Pubmed|22383849]

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genes

[gene|FE034FDCEAD4F4E91FD0147E16685632069E3316|yhfP]-[gene|1469148C83B1704E721652203EA31F0E2C33FDC6|yhfQ]

description

[Pubmed|22383849]

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Biological materials

Mutant

MGNA-A715 (yhfP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/715 NBRP B. subtilis, Japan]

BKE10320 (Δ[gene|FE034FDCEAD4F4E91FD0147E16685632069E3316|yhfP]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCGGCACACTCCTTTTC, downstream forward: _UP4_TAACAGGATCAGCTTGCAGA

BKK10320 (Δ[gene|FE034FDCEAD4F4E91FD0147E16685632069E3316|yhfP]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10320 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCGGCACACTCCTTTTC, downstream forward: _UP4_TAACAGGATCAGCTTGCAGA